Health Devices & Products

The Ultimate Guide on HPV DNA Diagnostic Kit (HPV 13+2)

Background of HPV DNA Diagnostic Kit (HPV 13+2)

The most common sexually transmitted infection (STI) globally is the human papillomavirus (HPV). The human papillomavirus (HPV) infection is now recognized as the leading cause of cervical cancer in women.

HPV DNA Diagnostic Kit

Figure 1. Human Papillomavirus (HPV) DNA Crude Prevalence and HR-HPV Type-specific Prevalence Among Women with Normal Cytology by World Region

Most HPV infections don’t lead to cancer. However, some kinds of genital HPV can cause cancer in the lower uterus, which attaches to the vaginal canal (cervix). Other malignancies have been associated with HPV infection, including cancers of the anus, penis, vagina, vulva, and back of the neck (oropharyngeal). These infections are often transmitted sexually or through other contagious skin diseases.

Oceania and Africa have the highest HPV prevalence, followed by Europe, America, and Asia. The use of different methods for detecting HPV DNA could play a role in the concerning HPV prevalence.

HPV DNA Diagnostic Kit

Figure 2. Sansure HPV DNA Diagnostic Kit (HPV 13+2)


The Medical Value of HPV DNA Diagnostic Kit (13+2) and the Principle Behind

HPV DNA Diagnostic Kit (13+2) can detect 15 kinds of high-risk genotypes, including HPV 16 and 18, while it can differentiate HPV16 and 18. It is applicable for cervical carcinoma screening throughout the world.

Significance of HPV DNA Diagnostic Kit (HPV 13+2)

HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59,66 and 68 are high-risk HPV types that are relatively prevalent around the globe. Types 16 and 18 are particularly malicious, since they are responsible for up to 70% of cervical malignancies in women, to which high importance must be attached. WHO guideline specifically refers to partial genotyping (e. the detection of HPV16 and 18 versus other carcinogenic types) to identify women at the highest risk of cervical cancer among those testing positive for HPV. Not only does the Human Papillomavirus DNA Diagnostic Kit (PCR-Fluorescence Probing) of Sansure cover 15 HPV types mentioned above and satisfy the global market’s demand on HPV screening, but it also differentiates HPV 16 and 18 detection, thereby responding to WHO initiative.

HPV DNA Diagnostic Kit



  • Principle of HPV DNA Diagnostic Kit


  • By applying real-time fluorescence quantitative PCR technology, this diagnostic kit uses several pairs of specific primers and fluorescence probes which are designed to target E nucleic acid conserved sequences of the above-mentioned 15 kinds of genotypes, accompanied with other ingredients in High-risk HPV-PCR mix, to achieve fast detection of HPV DNA, and detection of the 15 types of virus specimens and subtype identification of HPV16 and HPV18, through the changes of the four fluorescent signals (FAM, HEX/VIC, ROX, CY5). In addition, the PCR detection system uses the UNG enzyme + dUTP contamination-proof system to fully degrade possible PCR amplified products to avoid a false-positive result.


  • The PCR detection system uses a positive control to monitor β-globin in human epidermal cells to evaluate the amplification reaction of specimens and monitor the presence of PCR inhibitors.


  • The FAM channel of this diagnostic kit is used to test HPV 18-DNA, the HEX channel to test β-globin, ROX channel to test HPV 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68-DNA, and CY5 to test HPV 16-DNA.


There is evidence that HPV DNA testing can be used as a viable alternative to cytology screening, especially for nations that have not yet begun to invest resources and effort in establishing cytology-based screening programs.

Sansure HPV diagnostic kit has proved effective for screening and risk evaluation of cervical cancer by eliminating the problems of undertaking a diagnostic test for females. With HPV 13+2, one can easily and safely detect 15 of the most occurring and high-risk HPV genotypes. There are also several benefits for the customers, such as saving time, labor, and costs. There is no need to worry about inaccurate test results caused by small samples.

For more information, please visit Sansure at

Murtaza Ali

Murtaza Ali is a digital marketing expert and creative content writer with skills in online writing, blogging, and social media marketing. He likes to share his knowledge with readers in an inspiring and motivational way.

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